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Functional Genomics and Epidemiology of ESKAPE Pathogens Prof. Dr. rer. nat. Javid Iqbal Dasti was selected by German Research Foundation ( DFG) to work under the Supervision of Prof. Dr. med. Uwe Gross in the Institute of Medical Microbiology, University of Goettingen for his doctoral work. Project focused on the functional genomics of C. jejuni, led to the establishment of transposon mutagenesis. During his post doctoral work at University of Goettingen, novel genes of C. jejuni related to motility, chemotaxis and invasion were identified. In year 2009, Dr. Dasti joined Center for Chronic Immunodeficiency (CCI), Medical Center-University of Freiburg and worked for one year in close collaboration with Prof. Dr. Patrick Trieu-Cuot from Pasture institute Paris, under the mentorship of Prof. Dr. med. Philipp Henneke. An independent project written by Dr. Dasti focusing on the multiple aspects of C. jejuni pathogenesis was submitted to DFG that was awarded in year 2009, as a part of research consortium, that led to close collaboration with Prof. Dr. rer. nat. Stefan Bereswill in the Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, from year 2010 to 2011. Dr. Dasti moved back to Pakistan and joined Department of Microbiology, Quaid-i-Azam University Islamabad as an Assistant Professor. His work in Quaid-i-Azam University mostly focused on virulence and drug resistance of ESKAPE pathogens that led to the collaborations, joint publications and student exchange with Prof. Dr. Betsy Foxman, Professor of Epidemiology and director of the Center for Molecular and Clinical Epidemiology of Infectious Diseases at the University of Michigan, USA. Also very close collaboration was established with Prof. Dr. Simon C Andrews, Professor of Molecular Microbiology in the School of Biological Sciences (SBS) at the University of Reading, United Kingdom that led to student exchange and several joint publications supported by Commonwealth split-site PhD program. Personal interest of Dr. Dasti in virology initiated some research work and led to the collaboration and student exchange with Honorable Nobel laureate Prof. Charles M Rice in the Rockefeller University and Prof Jaccob George, Storr Professor of Hepatic Medicine at the Storr Liver Centre, Westmead Institute for Medical Research, University of Sydney. Dr. Dasti published his work in leading research journals including BMC infectious disease, Infection and Drug resistance, Journals of Microbiology Society of the United Kingdom. Most of his research work at QAU is supported by collaborative research grants. Focus of the current research is epidemiology of ESKAPE pathogens. |
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Selected Publications
Usman M, Marcus A, Fatima A, Aslam B, Zaid M, Khattak M, Bashir S, Masood S, Rafaque Z, Dasti JI. Synergistic Effects of Gentamicin, Cefepime, and Ciprofloxacin on Biofilm of Pseudomonas aeruginosa. Infect Drug Resist. 2023 Sep 6;16:5887-5898. doi: 10.2147/IDR.S426111. PMID: 37692466; PMCID: PMC10485136. JCR IF 4.33
Noreen A, Masood H, Zaib J, Rafaque Z, Fatima A, Shabbir H, Alam J, Habib A, Noor S, Dil K, Dasti JI. Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry Associated Escherichia coli Isolated from Retail Chicken Meat. Antibiotics (Basel). 2022 Nov 19;11(11):1663. doi: JCR IF 5.7 IMTIAZ, W., SYED, Z., RAFAQUE, Z., ANDREWS, S. C. & DASTI, J. I. 2021. Analysis of Antibiotic Resistance and Virulence Traits (Genetic and Phenotypic) in Klebsiella pneumoniae Clinical Isolates from Pakistan: Identification of Significant Levels of Carbapenem and Colistin Resistance. Infect Drug Resist, 14, 227. JCRIF 4.323 IMTIAZ, W., DASTI, J. I. & ANDREWS, S. C. 2021. Draft genome sequence of a carbapenemase-producing (NDM-1) and multidrug-resistant, hypervirulent Klebsiella pneumoniae ST11 isolate from Pakistan, with a non-hypermucoviscous phenotype associated with rmpA2 mutation. J Glob Antimicrob Resist, 25, 359-362. JCRIF 3.611 RAFAQUE, Z., ABID, N., LIAQAT, N., AFRIDI, P., SIDDIQUE, S., MASOOD, S., KANWAL, S. & DASTI, J. I. 2020. In-vitro Investigation of Antibiotics Efficacy Against Uropathogenic Escherichia coli Biofilms and Antibiotic Induced Biofilm Formation at Sub-Minimum Inhibitory Concentration of Ciprofloxacin. Infect Drug Resist, 13, 2801. JCRIF 4.332 JALIL, I., ARSHAD, M., KHAN, S. & DASTI, J. I. 2020. PNPLA3 and TM6SF2, but Not MBOAT7, Are Associated with Steatosis and HBV Viral Persistence in Pakistani Population. Jundishapur J Microbiol, 13. JCRIF 1.499 JALIL, I., ARSHAD, M., KHAN, S. & DASTI, J. I. 2020. The STAT4 and not the IFNL3 variant is associated with hepatitis B virus clearance in a population from the Khyber Pakhtunkhwa region of Pakistan. Arab J Gastroenterol, 21, 91-94. JCRIF 1.541 ALI, I., RAFAQUE, Z., AHMED, I., TARIQ, F., GRAHAM, S. E., SALZMAN, E., FOXMAN, B. & DASTI, J. I. 2019. Phylogeny, sequence-typing and virulence profile of uropathogenic Escherichia coli (UPEC) strains from Pakistan. BMC Infect Dis, 19, 1-9. JCRIF 3.401 ARSHAD, M., JALIL, I., RAZA, A., MALIK, S. & DASTI, J. I. 2019. Novel polymorphism in the promoter region of HLA-DQB1 is a predictor of anti-HCV therapy response. Jundishapur J Microbiol, 12. JCRIF 1.499 RAFAQUE, Z., DASTI, J. I. & ANDREWS, S. C. 2018. Draft genome sequence of a multidrug-resistant CTX-M-15 β-lactamase-producing uropathogenic Escherichia coli isolate (ST131-O25b-H30) from Pakistan exhibiting high potential virulence. J Glob Antimicrob Resist, 15, 164-165. JCRIF 3.611 ALI, I. & DASTI, J. I. 2018. Chikungunya virus; an emerging arbovirus in Pakistan. J Pak Med Assoc, 68, 252-7. JCRIF 0.929 KAUSAR, A., ANWAR, S., SIDDIQUE, N., AHMED, S. & DASTI, J. I. 2018. Prevalence of avian influenza H9N2 virus among wild and domesticated bird species across Pakistan. Pak J Zool, 50, 1347-1354. JCRIF 0.768 NAEEM, A. H., MUMTAZ, S., HALEEM, A., QAZI, M. A., MALIK, Z. A., DASTI, J. I. & AHMED, S. 2017. Isolation and molecular characterization of biosurfactant-producing bacterial diversity of Fimkassar oil field, Pakistan. Arab J Sci Eng, 42, 2349-2359. JCRIF 1.71 ALI, I., RAFAQUE, Z., AHMED, S., MALIK, S. & DASTI, J. I. 2016. Prevalence of multi-drug resistant uropathogenic Escherichia coli in Potohar region of Pakistan. Asian Pac J Trop Biomed, 6, 60-66. JCRIF 1.545 DASTI, J. I. 2016. Zika virus infections: an overview of current scenario. Asian Pac J Trop Med, 9, 621-625. JCRIF 2.285 JALIL, I., ARSHAD, M., RAFAQUE, Z., RAZIQ, F., WAZIR, R., MALIK, S. & DASTI, J. I. 2016. Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan. Asian Pac J Trop Biomed, 6, 609-613. JCRIF 1.545 ULLAH, R. W., ZAHUR, A. B., LATIF, A., DASTI, J. I., IRSHAD, H., AFZAL, M., RASHEED, T., MALIK, A. R. & QURESHI, Z.-U.-A. 2016. Detection of peste des petits ruminants viral RNA in fecal samples of goats after an outbreak in Punjab province of Pakistan: A longitudinal study. Biomed Res Int, 2016. JCRIF 3.362 ALSUHAIBANI, E., KIZILBASH, N., MALIK, S., DASTI, J., AL BELADI, F. & EL-MORSHEDI, N. 2016. Polymorphisms in promoter regions of IL-6 and IL-10 genes in breast cancer: a case-control study. Genet Mol Res, 15, 56. JCRIF 0.765 ALI, I., RAFAQUE, Z., AHMED, S., MALIK, S. & DASTI, J. I. 2016. Prevalence of multi-drug resistant uropathogenic Escherichia coli in Potohar region of Pakistan. Asian Pac J Trop Biomed, 6, 60-66. JCRIF 1.545 ULLAH, S., DASTI, J. I. & MALIK, S. 2015. Descriptive epidemiology of hereditary musculoskeletal and limb defects in the isolated population of Chitral, North-West Pakistan. Res J Med Sci, 31, 1047. JCRIF 0.754 ULLAH, R. W., ZAHUR, A. B., LATIF, A., IQBAL, J., ZAHRA, D. R. & SAEED-UL-HASSAN, K. 2015. Mild form of peste des petits ruminants virus (PPRV) in Pakistan. Pak J Zool, 47. JCRIF 0.768 FAHIM, H., DASTI, J. I., ALI, I., AHMED, S. & NADEEM, M. 2014. Physico-chemical analysis and antimicrobial potential of Apis dorsata, Apis mellifera and Ziziphus jujube honey samples from Pakistan. Asian Pac J Trop Biomed, 4, 633-641. JCRIF 1.545 BERESWILL, S., FISCHER, A., PLICKERT, R., HAAG, L.-M., OTTO, B., KüHL, A. A., DASTI, J. I., ZAUTNER, A. E., MUñOZ, M. & LODDENKEMPER, C. 2011. Novel murine infection models provide deep insights into the “menage a trois” of Campylobacter jejuni, microbiota and host innate immunity. PLoS One, 6, e20953. JCRIF 3.788 TAREEN, A. M., DASTI, J. I., ZAUTNER, A. E., GROß, U. & LUGERT, R. 2011. Sulphite: cytochrome c oxidoreductase deficiency in Campylobacter jejuni reduces motility, host cell adherence and invasion. Microbiology, 157, 1776-1785. JCRIF 2.871 DASTI, J. I., TAREEN, A. M., LUGERT, R., ZAUTNER, A. E. & GROß, U. 2010. Campylobacter jejuni: a brief overview on pathogenicity-associated factors and disease-mediating mechanisms. Int J Med Microbiol, 300, 205-211. JCRIF 4.768 TAREEN, A. M., DASTI, J. I., ZAUTNER, A. E., GROß, U. & LUGERT, R. 2010. Campylobacter jejuni proteins Cj0952c and Cj0951c affect chemotactic behaviour towards formic acid and are important for invasion of host cells. Microbiology, 156, 3123-3135. JCRIF 2.871 DASTI, J.I., GROß, U., POHL, S., LUGERT, R., WEIG, M. AND SCHMIDT-OTT, R. 2007. Role of the plasmid-encoded tet (O) gene in tetracycline-resistant clinical isolates of Campylobacter jejuni and Campylobacter coli. J Med Microbiol, 56, 833-837. JCRIF 2.836
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Conferences: Candida and Candidiasis today: Where are we, and where to go? (IFOCAN) 2005, Germany. September 25-28, 2005. Annual Meeting of German Society for Hygiene and Microbiology and the Association for General and Applied Microbiology (VAAM), Germany. Horizons in Molecular Biology. Decoding Nature: Hierarchy of Interactions. March 17-19, 2005. Goettingen, Germany. October 4th 2006, Annual Meeting of German Society for Hygiene and Microbiology, Germany. September, 2-5. 2007, 14th International workshop on Campylobacter, Helicobacter and Related Organisms, Zoonosis and public health, Rotterdam, the Netherlands. October 30th - November 4 2007, Annual Meeting of German Society for Hygiene and Microbiology and Federation of European Microbiological Societies, Germany. Annual scientific meeting Indonesian association of clinical microbiology 17-27 March 2008, Indonesia. 25th Symposium in Jena, 2-3, June 2008, Campylobacter infection, Germany (verbal presentation). Flow-jo-Seminar 23. April, 2009, University of Freiburg, Germany 2010, WHO Symposium Hannover, Germany March 26- 30, 2012, exploring the hidden potential of Microorganisms, German Academic Exchange Service (DAAD), and Pakistan. February 22-23, 2013 10th Annual Conference of Infectious Diseases, Pakistan. 1st international conference on Biotechnology, 21-25th April 2013 at National Institute of Biotechnology and Genetic Engineering, Faisalabad Pakistan. Poster and verbal presentation at 20th annual meeting by Medical Research Society of Pakistan, 25th May 2013 at Shawkat Khanum Memorial Cancer Hospital Lahore Pakistan. Arranged a one-day symposium on Blood Donation “Donate to the deserving” at 8 October 2013, at Quaid-i-Azam University Islamabad. Poster presentation at 11th annual conference on infectious disease, by Medical Microbiology and Infectious Diseases Society of Pakistan, at 14-15th March 2014 Hotel Margala, Islamabad. Research data (Abstract submitted w/o participation) at 24th ECCMID (European Congress of Clinical Microbiology and Infectious diseases by ESCMID (European Society of Clinical Microbiology and Infectious Diseases) 10-13 th May 2014 at Barcelona Spain. From 10-13th May 2014, New Challenges and Frontiers in infectious diseases, Islamabad, Pakistan (Posters & verbal presentations) From 30 August to 4 September 2014, Session: CSI-02_Inflammation & disease (Poster), FEBS EMBO 2014 Conference, Paris, France International Conference on Medical Biological and Pharmaceutical Sciences (ICMBPS), Berlin, Germany, November 29, 2015 (verbal presentations). Poster presentation at first international conference on advancements in Biotechnology 30th -31st March 2016 at GC Women University Faisalabad. International symposium on Nano medicines: Development, Testing, and Application possibilities organized by Nanotheranostics Lab, Nuclear Medicine Oncology and Radiotherapy Institute, Pakistan Atomic Energy Commission Islamabad on 1st March 2016. One-day symposium on Burden of Antibiotic Resistance in Neonates Quaid-e-Azam University, Islamabad 29 March 2016 One-day symposium on the theme of Frontiers in Biology: From Discovery to Applications, Allama Iqbal Open University, Islamabad, 9 Jan 2017 Presenting poster of M.Phil. Research work entitled “Efficacy of ceftazidime and Trimethoprim on planktonic cells and biofilms of Uropathogenic E.coli” at 11th International Biennial Conference of Pakistan Society of Microbiology 18-20 Dec 2018. International conference on applied zoology, Quaid-i-Azam University (QAU) Quaid-i-Azam University, 25th Nov 2021
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Scientific Projects and Funding Commonwealth Research Support; 2019-2020; University of Reading, United Kingdom Commonwealth Research Support; 2016-2017; University of Reading, United Kingdom University Indigenous Research Fund, Year 2012-2018 URF-QAU 2012-2018 IPFP, HEC, Pakistan Deciphering Molecular Mechanisms of virulence of Campylobacter infection, University of Göttingen, Germany (Funded by DFG). To establish in vitro model airways to investigate the role of Toll-like receptors in mucociliary clearance and pneumococcal infection, University of Freiburg, Germany (Funded by DFG). To investigate Interplay of TLR2 and lipoproteins in group B streptococcal sepsis, University of Freiburg, Germany (Funded by NIH, USA in collaboration with Pasteur Institute, Paris, France Identification and characterization of Campylobacter jejuni factors relevant for the infection process, University of Göttingen, Germany (Funded by DFG, via GRK335).
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